金胺O

化合物

金胺O是一種二芳基甲烷螢光染料，常態下為黃色針狀結晶。極易溶於水，易溶於乙醇。

Auramine O

IUPAC名
bis[4-(dimethylamino)phenyl]methaniminium chloride

别名

Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C.I. 41000

识别

[N@H]=C(c1ccc(N(C)C)cc1)c2ccc(N(C)C)cc2

1/C17H21N3/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4/h5-12,18H,1-4H3

JPIYZTWMUGTEHX-UHFFFAOYAY

性质

C₁₇H₂₂ClN₃

303.83 g·mol⁻¹

267 °C

危险性

R：R22 R24 R40

若非注明，所有数据均出自标准状态（25 ℃，100 kPa）下。

金胺O可用於抗酸細菌（例如Mycobacterium）, 其方法類似抗酸染色法。^[1] 也被當做希夫試劑的螢光版來使用。^[2]

在金胺-若丹明染色法中與若丹明B一起用來給結核桿菌染色。^[3]^[4] 同時也可用作防腐劑。

參考文獻编辑

^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L. Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis. Hum Pathol. 1984, 15 (11): 1085–9. PMID 6208117. doi:10.1016/S0046-8177(84)80253-1.
^ Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B. Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli. Gastroenterology. 1980, 78 (4): 782–90. PMID 6986320.
^ Truant J, Brett W, Thomas W. Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine. Henry Ford Hosp Med Bull. 1962, 10: 287–96. PMID 13922644.
^ Arrowood M, Sterling C. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J Clin Microbiol. 1989, 27 (7): 1490–5. PMC 267601  . PMID 2475523.

外部連結编辑

Auramine O spectra data （页面存档备份，存于互联网档案馆）

取自“https://zh.wikipedia.org/w/index.php?title=金胺O&oldid=67770403”