外毒素A

外毒素A(英語:exotoxin A)也稱绿脓杆菌外毒素假单胞菌外毒素(Pseudomonas exotoxin),是由綠膿桿菌(学名:铜绿假单胞菌,Pseudomonas aeruginosa)产生的外毒素[1]。它的功能是将NAD+上的二磷酸腺苷核糖(ADP-ribose)转移到目标蛋白质上,这一过程称为ADP-核糖基化修饰。它的致病机制是通过将真核延伸因子2(EF2)的白喉酰胺英语Diphthamide残基ADP-核糖基化来抑制EF2[2],从而导致多肽的延长停止(毒素的机制类似于白喉毒素)。目前该现象已被用于乙肝[3]和癌症[4]的治疗研究。

外毒素A二聚體,铜绿假单胞菌

参考文献编辑

  1. ^ Yates, Susan P.; Taylor, Patricia L.; Jørgensen, René; Ferraris, Dana; Zhang, Jie; Andersen, Gregers R.; Merrill, A. Rod. Structure-function analysis of water-soluble inhibitors of the catalytic domain of exotoxin A from Pseudomonas aeruginosa. The Biochemical Journal. 2005-02-01, 385 (Pt 3): 667–675 [2018-08-14]. ISSN 1470-8728. PMID 15458385. doi:10.1042/BJ20041480. (原始内容存档于2018-08-09). 
  2. ^ Yates, Susan P.; Merrill, Allan R. Elucidation of eukaryotic elongation factor-2 contact sites within the catalytic domain of Pseudomonas aeruginosa exotoxin A. The Biochemical Journal. 2004-05-01, 379 (Pt 3): 563–572 [2018-08-14]. ISSN 1470-8728. PMID 14733615. doi:10.1042/BJ20031731. (原始内容存档于2018-08-09). 
  3. ^ Hafkemeyer, Peter; Brinkmann, Ulrich; Brinkmann, Elizabeth; Pastan, Ira; Blum, Hubert-E.; Baumert, Thomas-F. Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells. World Journal of Gastroenterology. 2008-05-14, 14 (18): 2810–2817 [2018-08-14]. ISSN 1007-9327. PMID 18473403. doi:10.3748/wjg.14.2810. (原始内容存档于2018-08-09). 
  4. ^ Stuckey, Daniel W.; Hingtgen, Shawn D.; Karakas, Nihal; Rich, Benjamin E.; Shah, Khalid. Engineering toxin-resistant therapeutic stem cells to treat brain tumors. Stem Cells (Dayton, Ohio). 2015-2, 33 (2): 589–600 [2018-08-14]. ISSN 1549-4918. PMID 25346520. doi:10.1002/stem.1874. (原始内容存档于2018-08-09).