花菁(英語:Cyanines,或譯為青色素花青青藍)是一大類熒光染料[1],儘管其名字中帶「青」字,但其電磁波譜紫外(UV)覆蓋的近紅外(NIR)。

Cy3和Cy5染料的結構
探針 激發波長 發射波長 分子量 量子產率
Cy2 489 506 714 QY 0.12
Cy3 550 570 767 QY 0.15 [2]
Cy3B 558 572 658 QY 0.67
Cy3.5 591 604 1102 QY 0.15
Cy5 646 662 792 QY 0.27[2]
Cy5.5 675 694 1128 QY 0.28[3]
Cy7 743 767 818 QY 0.28

參考文獻

編輯
  1. ^ Kvach, Maksim V.; Ustinov, Alexey V.; Stepanova, Irina A.; Malakhov, Andrei D.; Skorobogatyi, Mikhail V.; Shmanai, Vadim V.; Korshun, Vladimir A. A Convenient Synthesis of Cyanine Dyes: Reagents for the Labeling of Biomolecules. European Journal of Organic Chemistry. 2008, 2008 (12): 2107–2117 [2022-11-02]. ISSN 1099-0690. doi:10.1002/ejoc.200701190. (原始內容存檔於2022-10-28) (英語). 
  2. ^ 2.0 2.1 Mujumdar B, Ernst A, Mujumdar SR, Lewis CJ, Waggoner AS. Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters. Bioconjugate Chemistry. Mar 1993, 4 (2): 105–111. PMID 7873641. doi:10.1021/bc00020a001. 
  3. ^ Umezawa K, Matsui A, Nakamura Y, Citterio D, Suzuki K. Bright, color-tunable fluorescent dyes in the Vis/NIR region: establishment of new "tailor-made" multicolor fluorophores based on borondipyrromethene. Chemistry. 2009, 15 (5): 1096–106. PMID 19117043. doi:10.1002/chem.200801906.