蚓激酶
蚓激酶（Lumbrokinase）是一种纤溶酶[1][注 1]，大小介于25-32kDa之间[2]，可以多种途径促进纤维蛋白的溶解，除了具有纤溶酶活性外，还可作为纤维蛋白溶酶原激活物以刺激纤溶酶的生成，又可促进组织间其他纤维蛋白溶酶原激活物的活性[3]。蚓激酶最早于1991年在正蚓属蚯蚓Lumbricus bimastus体内发现[2][4]，亦存在于赤子爱胜蚓、粉正蚓与等多种蚯蚓的体腔与消化道内，这种纤溶酶结构稳定，且在人体中可被小肠吸收，因而有作为抗血栓药物的潜力[5]，且不同于链激酶等其他种专一性较低的抗血栓药物，蚓激酶对纤维蛋白的专一性很高，只有纤维蛋白存在时才具有酵素活性，而较无造成纤溶亢进导致大出血之虞[2][6]。蚓激酶已在临床上被用于治疗中风与其他心血管疾病[2]。
注释 编辑
参考资料 编辑
- ^ Hu, R. Codon optimization, expression, and characterization of recombinant lumbrokinase in goat milk. Protein Expression and Purification. 2004, 37 (1): 83–8. PMID 15294284. doi:10.1016/j.pep.2004.05.018.
- ^ 2.0 2.1 2.2 2.3 Wang YH, Chen KM, Chiu PS, Lai SC, Su HH, Jan MS, Lin CW, Lu DY, Fu YT, Liao JM, Chang JT, Huang SS. Lumbrokinase attenuates myocardial ischemia-reperfusion injury by inhibiting TLR4 signaling. Journal of Molecular and Cellular Cardiology. 2016, 99: 113-122. doi:10.1016/j.yjmcc.2016.08.004.
- ^ GangQiang Li, Kevin Yueju Wang, DaHui Li, Nan Wang, DeHu Liu. Cloning, Expression and Characterization of a Gene from Earthworm Eisenia fetida Encoding a Blood-Clot Dissolving Protein. PLoS One: e53110. doi:10.1371/journal.pone.0053110.
- ^ Hisashi MIHARA, Hiroyuki SUMI, Tomoyuki YONETA, Hideaki MIZUMOTO, Ryuzo IKEDA, Masao SEIKI, Masugi MARUYAMA. A Novel Fibrinolytic Enzyme Extracted from the Earthworm, Lumbricus rubellus. The Japanese Journal of Physiology: 461-472. doi:10.2170/jjphysiol.41.461.
- ^ Ge, T.; Sun, Z.; Fu, S.; Liang, G. Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast. Protein Expression and Purification. 2005, 42 (1): 20–8. PMID 15927482. doi:10.1016/j.pep.2005.04.005.
- ^ Edwin L.Cooper, M.Balamurugan. Unearthing a source of medicinal molecules. Drug Discovery Today. 2010, 15 (21-22): 966-972. doi:10.1016/j.drudis.2010.09.004.