HT-29

HT-29是广泛应用于生物学和癌症研究的人类结肠癌细胞系，最初于1964年从一名44岁的白人女性患者分离出来的^[1]。

特征

细胞中过度表达的p53基因

HT-29细胞形成紧密的单层膜，同时表现出与小肠上皮细胞的相似性。HT-29细胞过度表达p53基因，但p53基因在273位拥有突变，导致组氨酸取代了精氨酸的位置。HT-29会在含有苏拉明的培养基中快速增殖，并且具有相应的c-myc癌基因高度表达。c-myc基因若然被解除管制（deregulated），则可能与HT-29细胞的生长因子需求有关^[2]。

科研用途

在临床前研究中，已经研究了HT-29细胞在体外分化及模拟真实结肠组织的能力。这特性使HT-29细胞可应用于上皮细胞研究^[3]，还可以在体内进行测试。HT-29细胞在细胞培养物中以半乳糖替代葡萄糖的位置，并且添加丁酸酯或酸来进行终末分化，分化为肠上皮细胞，从而可以研究其细胞分化途径及其对周围环境的依赖性^[1]。对HT-29细胞的研究表明，归因于毛喉素、秋水仙素、诺考达唑和紫杉醇诱导的分化^[4]，半乳糖介导的细胞分化会增强黏着小带^[5]。

培养

尽管HT-29细胞可以在缺乏生长因子的细胞培养基中增殖，但其倍增时间约为4天，而在培养基中添加胎牛血清，可以将HT-29细胞的倍增时间减少至1天^[2]。HT-29细胞具有很高的葡萄糖消耗，并且在含有25 mM葡萄糖和10%血清的标准培养基中并未出现细胞分化^[1]。

参考资料

^ ^1.0 ^1.1 ^1.2 Martínez-Maqueda, D; et al. HT29 Cell Line. The Impact of Food Bioactives on Health. Springer. 2015: 113–124. ISBN 978-3-319-15791-7. PMID 29787047. doi:10.1007/978-3-319-16104-4_11.
^ ^2.0 ^2.1 Coudray, Anne-Marie; et al. Proliferation of the Human Colon Carcinoma Cell Line HT29: Autocrine Growth and Deregulated Expression of the c-myc Oncogene (PDF). Cancer Research. 1 December 1989, 49: 6566–6571 [2020-01-01]. （原始内容存档 (PDF)于2020-01-01）.
^ Hirn, M; et al. HT-29 cells are an in vitro model for the generation of cell polarity in epithelia during embryonic differentiation. Proceedings of the National Academy of Sciences. 1988, 85 (1): 136–140. PMC 279498  . PMID 3277169. doi:10.1073/pnas.85.1.136.
^ Cohen, E; et al. Induced differentiation in HT29, a human colon adenocarcinoma cell line. Journal of Cell Science. August 1999, 112: 2657–2666. PMID 10413674.
^ Gout, S; et al. Early enterocytic differentiation of HT-29 cells: biochemical changes and strength increases of adherens junctions. Experimental Cell Research. 1 October 2004, 299 (1): 498–510. PMID 15350547. doi:10.1016/j.yexcr.2004.06.008.

外部链接

Cellosaurus entry for HT-29（页面存档备份，存于互联网档案馆）

[one-1] 1.0 ^1.1 ^1.2 Martínez-Maqueda, D; et al. HT29 Cell Line. The Impact of Food Bioactives on Health. Springer. 2015: 113–124. ISBN 978-3-319-15791-7. PMID 29787047. doi:10.1007/978-3-319-16104-4_11.

[two-2] 2.0 ^2.1 Coudray, Anne-Marie; et al. Proliferation of the Human Colon Carcinoma Cell Line HT29: Autocrine Growth and Deregulated Expression of the c-myc Oncogene (PDF). Cancer Research. 1 December 1989, 49: 6566–6571 [2020-01-01]. （原始内容存档 (PDF)于2020-01-01）.

[3] Hirn, M; et al. HT-29 cells are an in vitro model for the generation of cell polarity in epithelia during embryonic differentiation. Proceedings of the National Academy of Sciences. 1988, 85 (1): 136–140. PMC 279498  . PMID 3277169. doi:10.1073/pnas.85.1.136.

[4] Cohen, E; et al. Induced differentiation in HT29, a human colon adenocarcinoma cell line. Journal of Cell Science. August 1999, 112: 2657–2666. PMID 10413674.

[5] Gout, S; et al. Early enterocytic differentiation of HT-29 cells: biochemical changes and strength increases of adherens junctions. Experimental Cell Research. 1 October 2004, 299 (1): 498–510. PMID 15350547. doi:10.1016/j.yexcr.2004.06.008.

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