BNL 1ME A.7R.1
BNL 1ME A.7R.1是小鼠肝肿瘤细胞系，最初是正常小鼠胚胎的非致瘤性肝上皮细胞BNL CL.2，经过致癌的甲基胆蒽 (3-methylcholanthrene) 诱导后转化而建立的[1]。新建立的细胞与BNL CL.2细胞相比，E-钙粘蛋白的信使核糖核酸和蛋白水平均显著降低，而骨髓间质标记物和相关转录因子的表达水平则提高[2]。新建立的细胞可以在0.3%的琼脂培养基中生长及增殖，并且对鼠痘病毒呈阴性。有科学家将此癌细胞通过外科手术直接植入近交野生型BALB/c小鼠的肝脏，希望能够阐明固体器官癌转移的生物学机制[3]。有研究指出生育三烯酚能够降低BNL 1ME A.7R.1细胞的活性，并且导致细胞凋亡[4]。
参考资料
编辑- ^ Patek, PQ; Collins, JL; Cohn, M. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis.. Nature. 1978-11-30, 276 (5687): 510–1 [2019-12-08]. PMID 723934. doi:10.1038/276510a0.
- ^ Oh, J; Kwak, JH; Kwon, DY; Kim, AY; Oh, DS; Je, NK; Lee, J; Jung, YS. Transformation of Mouse Liver Cells by Methylcholanthrene Leads to Phenotypic Changes Associated with Epithelial-mesenchymal Transition.. Toxicological research. 2014-12, 30 (4): 261–6 [2019-12-08]. PMID 25584145. doi:10.5487/TR.2014.30.4.261.
- ^ Das, DK; Durojaiye, V; Ilboudo, A; Naidoo, MK; Ogunwobi, O. A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis.. Journal of visualized experiments : JoVE. 2015-10-24, (105): e52858 [2019-12-08]. PMID 26555484. doi:10.3791/52858.
- ^ Har, CH; Keong, CK. Effects of tocotrienols on cell viability and apoptosis in normal murine liver cells (BNL CL.2) and liver cancer cells (BNL 1ME A.7R.1), in vitro.. Asia Pacific journal of clinical nutrition. 2005, 14 (4): 374–80 [2019-12-08]. PMID 16326644.